Related Experiment Video
Updated: May 14, 2026
07:01
3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
Published on: October 24, 2019
Electron-microscopic and immunohistochemical study in Henoch-Schoenlein nephritis
Aldona Wozniak1, Katarzyna Pluta-Hadas, Jakub Zurawski
1Department of Clinical Pathomorphology, Karol Marcinkowski University of Medical Sciences, Poznan, Poland. aldona.wozniak@wp.pl
Ultrastructural Pathology
|February 7, 2013
Summary
Henoch-Schoenlein nephritis (HSN) is a common childhood kidney disease. Glomerulosclerosis and fibrosis predict poor outcomes, while decreased p27 expression indicates severity.
Area of Science:
- Pediatric Nephrology
- Renal Pathology
Background:
- Henoch-Schoenlein nephritis (HSN) is the most frequent secondary nephropathy in children.
- It can progress to end-stage renal disease in up to 20% of pediatric cases.
Purpose of the Study:
- To investigate prognostic markers in Henoch-Schoenlein nephritis (HSN).
- To evaluate the role of specific biomarkers in predicting HSN outcomes.
Main Methods:
- Retrospective review of 44 HSN cases (32 children, 12 adults).
- Light microscopy, electron microscopy (EM), and immunohistochemistry for Ki-67, PCNA, and p27.
- Analysis of glomerulosclerosis, interstitial fibrosis, and biomarker expression.
Main Results:
- Light microscopy revealed varying HSN grades (II, III, IV, VI).
- Glomerulosclerosis and interstitial fibrosis correlated with poor outcomes.
- Decreased p27 podocyte expression was associated with more severe HSN grades.
- No correlation found between Ki-67/PCNA mesangial expression and outcome.
Conclusions:
- Glomerulosclerosis and interstitial fibrosis are key prognostic indicators in HSN.
- Reduced p27 expression in podocytes signifies disease severity.
- EM aids in detecting early glomerulosclerosis in advanced HSN cases.
