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High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

High-Performance Liquid Chromatography: Instrumentation

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

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Updated: May 14, 2026

A Microfluidic Chip for ICPMS Sample Introduction

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Microfluidic integration of parallel solid-phase liquid chromatography.

Jens Huft¹, Charles A Haynes, Carl L Hansen

¹Centre for High-Throughput Biology, University of British Columbia, Vancouver BC, Canada.

Analytical Chemistry

|February 7, 2013

Summary

This summary is machine-generated.

A novel microfluidic chromatography system enables automated DNA and gene purification. This integrated device offers high recovery rates and promises advancements in on-chip genomics and diagnostics.

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Area of Science:

Biotechnology
Analytical Chemistry
Microfluidics

Background:

Microfluidic devices offer miniaturization and automation potential for complex analytical tasks.
Robust packing of high-performance separation columns in polydimethylsiloxane (PDMS) microfluidic devices remains a challenge.

Purpose of the Study:

To develop a fully integrated microfluidic chromatography system for automated sample processing.
To demonstrate the system's capability in DNA separation and gene purification.

Main Methods:

Utilized a novel column geometry for robust packing of separation columns in PDMS microfluidic devices.
Integrated valves using multilayer soft lithography (MSL) for on-chip plumbing.
Employed parallel high-performance separation columns for automated sample loading, gradient generation, separation, detection, and recovery.

Main Results:

Successfully separated fluorescently labeled DNA and purified variable regions of mouse immunoglobulin genes using strong anion exchange (AEX) resin.
Achieved parallel sample recovery in an immiscible oil stream, ensuring low dilution and high recovery rates.
Demonstrated nucleic acid size selection and recovery on subnanogram DNA samples.

Conclusions:

The developed microfluidic chromatography system provides a fully integrated solution for on-chip analysis.
The system's capabilities in DNA and gene purification hold significant promise for on-chip genomics applications.
Potential applications include sequencing library preparation, cloning, and sample fractionation for diagnostics.