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Related Concept Videos

PCR01:32

PCR

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Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
PCR - Polymerase Chain Reaction01:32

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The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Radical Chain-Growth Polymerization: Mechanism01:09

Radical Chain-Growth Polymerization: Mechanism

The radical chain-growth polymerization mechanism consists of three steps: initiation, propagation, and termination of polymerization. The polymerization initiates when a free radical generated from the radical initiator adds to the unsaturated bond in the monomer. The unpaired electron of the free radical and one π electron in the unsaturated bond creates a σ bond between the free radical and the monomer. As a result, the other π electron in the unsaturated bond converts this species into the...

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Updated: May 14, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Polymerase chain displacement reaction.

Claire L Harris1, Irma J Sanchez-Vargas, Ken E Olson

  • 1Oxitec Ltd, Abingdon, Oxfordshire, UK.

Biotechniques
|February 7, 2013
PubMed
Summary
This summary is machine-generated.

A new polymerase chain displacement reaction (PCDR) technique enhances quantitative PCR (qPCR) sensitivity by tenfold for viral diagnostics. This rapid, one-tube method improves nucleic acid detection sensitivity and speed.

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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

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Area of Science:

  • Molecular Biology
  • Virology
  • Biochemistry

Background:

  • Quantitative PCR (qPCR) is standard for viral diagnostics, requiring high sensitivity and specificity.
  • Detecting low viral genomic material concentrations is crucial for accurate diagnostics.

Purpose of the Study:

  • To introduce and evaluate a novel technique, polymerase chain displacement reaction (PCDR), for enhanced qPCR sensitivity.
  • To improve nucleic acid detection methods for viral diagnostics.

Main Methods:

  • Developed a rapid, capped, one-tube reaction utilizing multiple nested primers.
  • Incorporated a polymerase with strand displacement activity to displace extension strands from inner primers.
  • Tested PCDR on dengue virus sequences for proof-of-principle.

Main Results:

  • Achieved approximately tenfold increase in sensitivity compared to standard qPCR assays.
  • Demonstrated a greater than twofold increase in amplification product per cycle.
  • PCDR offers increased sensitivity and speed over conventional PCR.

Conclusions:

  • Polymerase chain displacement reaction (PCDR) significantly enhances qPCR sensitivity for viral diagnostics.
  • The PCDR method offers a promising advancement for sensitive nucleic acid detection.
  • Increased sensitivity in PCDR is valuable for detecting low viral loads.