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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Precise breakpoint localization of large genomic deletions using PacBio and Illumina next-generation sequencers

Michal J Okoniewski1, Janine Meienberg, Andrea Patrignani

  • 1Functional Genomics Center Zurich, Zurich, Switzerland. michal.okoniewski@fgcz.ethz.ch

Biotechniques
|February 7, 2013
PubMed

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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.