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Estrogens and Hyperglycemic Modulation of mRNAs Expressions Involved in Bone Metabolism: An Overshadowed Association?

Dalia Somjen1, Sara Katzburg, Orli Sharon

  • 1Institute of Endocrinology, Metabolism and Hypertension, Tel- Aviv Sourasky Medical Center; Tel- Aviv 64239, and the Sackler Faculty of Medicine, Tel- Aviv University , Tel- Aviv , ISRAEL.

Connective Tissue Research
|February 7, 2013
PubMed

Insights

High glucose levels impair estrogen

Area of Science:

  • Endocrinology
  • Bone Biology
  • Cellular Physiology

Background:

  • Estrogen receptors (ERα, ERβ), vitamin D receptor (VDR), and lipoxygenases (12LO, 15LO) are key in bone health.
  • Estrogen's protective effects on bone are less clear in diabetic women.
  • High glucose (HG) may interfere with these pathways in bone cells.

Purpose of the Study:

  • To investigate the impact of HG on gene expression and estrogen modulation in human bone cells (SaOS2).
  • To determine if HG affects ERα, ERβ, VDR, 1OHase, 12LO, and 15LO mRNA expression and their response to estrogenic compounds.

Main Methods:

  • SaOS2 cells were cultured in normal glucose (NG) and high glucose (HG) media.
  • Cells were treated with estradiol (E2), ERα agonist (PPT), and ERβ agonist (DPN).
  • mRNA expression of target genes and DNA synthesis were analyzed.

Main Results:

  • HG increased DNA synthesis and creatine kinase activity.
  • HG abolished estrogenic compound-induced DNA synthesis stimulation.
  • HG upregulated 12LO and 15LO mRNA, and to a lesser extent ERβ and VDR mRNA, while not affecting ERα or 1OHase mRNA.

Conclusions:

  • High glucose disrupts estrogen signaling pathways in bone cells.
  • HG alters the expression of key genes involved in bone metabolism and inflammation.
  • Further research is needed to clarify the mechanisms and implications for bone health in diabetes.

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