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Updated: May 14, 2026
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
Published on: February 24, 2021
Extended storage of autologous apheresis platelets in plasma
S J Slichter1, D Bolgiano, J Corson
1Puget Sound Blood Center, Seattle, WA 98104-1256, USA. sjslichter@psbc.org
Background And Objectives:
The purpose of our studies was to determine the effects of extended platelet storage on poststorage platelet viability.
Materials And Methods:
Normal subjects were recruited to donate platelets using two different apheresis systems: either the COBE Spectra (n = 58) or the Haemonetics MCS+ (n = 84). Platelet recovery and survival data from the two systems were compared with each other and with in vitro measurements of the stored platelets.
Results:
There were no significant differences in either platelet recoveries or survivals between the two machines between 1 and 8 days of storage. Combining the data from both machines, platelet recoveries decreased by 2.6% and survivals by 0.3 days/storage day. In vitro assays did not predict either platelet recoveries or survivals during storage for 5-8 days. After 9 days of storage, pHs were unacceptable (≤ 6.1), suggesting that 8 days will be the longest possible storage time.
Conclusions:
These data suggest that, if stored platelet bacterial contamination issues are resolved, significant extension of platelet storage times is possible.
