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Updated: May 14, 2026
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Published on: June 11, 2016
Quantification of the 16S-23S rRNA internal transcribed spacers of Burkholderia xenovorans strain LB400 using
M-P Norini1, C Secher, M Lollier
1EDBS, LVBE EA3991, University of Haute Alsace, Colmar Cedex, France. mariepaule.norini@outlook.com
Abstract:
This study establishes a new real-time PCR assay (using SYBR Green™ detection) for the identification and the direct quantification of specific individual Burkholderia xenovorans strain LB400 from DNA samples of soil and sediment. Specific primers were designed to amplify a 190-bp fragment of the 16S-23S rRNA internal transcribed spacers (ITS) from LB400. The specificity of primers was evaluated using 21 strains. The detection limit of the real-time PCR was analysed on soil samples inoculated with LB400 and was of 6 copies (10(5) CFU g(-1) of dry sample). The 16S-23S rRNA ITS primers developed in this work for rapid quantification of LB400 were validated. The assay allowed the quantification of LB400 as pure strain and among the indigenous microbial community in samples of soil and sediment (105-day experiment).
