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Quantitation of hexadecylphosphocholine by high-performance thin-layer chromatography with densitometry
1Institute of Pharmacology and Toxicology, University of Göttingen, F.R.G.
Journal of Chromatography
|January 26, 1990
Summary
This study presents a new method for measuring hexadecylphosphocholine (HePC), an anticancer drug, in plasma. The technique uses chromatography and densitometry, offering a simple approach for clinical drug monitoring.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Biochemistry
Background:
- Ether phospholipids play crucial roles in cellular functions.
- Hexadecylphosphocholine (HePC) is an antineoplastic ether phospholipid with therapeutic potential.
- Accurate quantification of HePC is essential for clinical applications and drug monitoring.
Purpose of the Study:
- To develop and validate a sensitive method for determining hexadecylphosphocholine (HePC) in biological samples.
- To establish a practical approach for clinical drug monitoring of HePC.
- To enable simultaneous analysis of HePC and endogenous phospholipids.
Main Methods:
- High-performance thin-layer chromatography (HPTLC) for lipid separation.
- Cupric sulphate charring for visualization.
- In situ densitometry for quantitative analysis.
- Ion-exchange chromatography and gel filtration for complex sample analysis.
Main Results:
- The method allows for the determination of HePC with a lower limit of approximately 25 pmol.
- Plasma samples can be analyzed directly from concentrated extracts without extensive clean-up.
- The technique facilitates the simultaneous study of endogenous phospholipids and HePC.
Conclusions:
- A robust and sensitive HPTLC-based method for HePC quantification has been established.
- This method is suitable for clinical drug monitoring due to its simplicity and efficiency.
- The developed approach supports comprehensive phospholipid analysis in biological matrices.