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Related Experiment Video

Updated: May 14, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

A fast analysis method to quantify nanoparticle uptake on a single cell level.

Adriano A Torrano1, Julia Blechinger, Christian Osseforth

  • 1Ludwig-Maximilians-University Munich, Department of Chemistry & Center for NanoScience, Butenandtstrasse 11, Gerhard-Ertl-Gebäude, 81377 Munich, Germany.

Nanomedicine (London, England)
|February 7, 2013
PubMed
Summary
This summary is machine-generated.

A new ImageJ macro, Particle_in_Cell-3D, enables precise measurement of nanoparticle uptake into cells. This method accurately quantifies particle numbers, advancing cell biology research.

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Last Updated: May 14, 2026

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14:53

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08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

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Area of Science:

  • Cell biology
  • Nanotechnology
  • Microscopy

Background:

  • Accurate quantification of particle uptake into cells is crucial for understanding cellular responses to nanomaterials.
  • Existing methods may lack precision or throughput for analyzing nanoparticle-cell interactions.

Purpose of the Study:

  • To develop and validate a novel method for the absolute quantification of nano- and micro-particle uptake into single cells.
  • To introduce Particle_in_Cell-3D, a freely available ImageJ macro for image analysis.

Main Methods:

  • Development of Particle_in_Cell-3D, an ImageJ macro for analyzing confocal fluorescence image stacks.
  • 3D reconstruction of single cells and segmentation into intracellular and membrane regions.
  • Particle localization, color-coding, and quantification based on calibrated particle intensity.

Main Results:

  • Successful application of Particle_in_Cell-3D to quantify 80-nm mesoporous silica nanoparticle uptake in HeLa cells.
  • Accurate quantification of 100-nm polystyrene nanoparticle agglomerate uptake, validated by stimulated emission depletion microscopy.
  • Demonstration of the macro's capability for absolute particle number determination.

Conclusions:

  • Particle_in_Cell-3D provides a fast and accurate approach for quantifying particle uptake into cells.
  • The method facilitates precise analysis of nanoparticle-cell interactions.
  • This tool enhances research in nanomedicine, toxicology, and cellular uptake studies.