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Updated: May 14, 2026

Murine Dermal Fibroblast Isolation by FACS

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

Bone marrow contributions to fibrosis.

Alison Mackinnon¹, Stuart Forbes

¹MRC Centre for Regenerative Medicine, University of Edinburgh, Scotland, UK.

Biochimica Et Biophysica Acta

|February 7, 2013

Summary

This summary is machine-generated.

Bone marrow cells can contribute to organ fibrosis after injury. Targeting specific recruitment factors may offer new therapies for pathological scarring.

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Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Published on: March 12, 2020

Area of Science:

Cell biology
Immunology
Pathology

Background:

Bone marrow-derived cells, including fibrocytes and myofibroblasts, circulate and home to injured organs.
These cells contribute to wound healing and collagen deposition, potentially leading to pathological fibrosis in chronic injury.
The contribution of circulating cells to organ fibrosis varies, with intrinsic cells dominating in some organs, while circulating cells are major players in others.

Purpose of the Study:

To investigate the functional contribution of bone marrow-derived cells to organ fibrosis.
To explore sophisticated methods for assessing the role of these cells in fibrotic responses.
To identify factors mediating fibrocyte recruitment and explore therapeutic targets for fibrosis.

Main Methods:

Utilizing labelled donor bone marrow for cell tracking experiments in mice.
Employing bone marrow transplantation with collagen reporter systems to assess transcriptional activity of bone marrow-derived cells.
Performing bone marrow transplants from donors with collagen mutations to evaluate functional differences in fibrosis.
Identifying and blocking factors like CXCL12 and CXCL16 that mediate fibrocyte recruitment.

Main Results:

Bone marrow-derived cells can home to injured organs and contribute to collagen deposition.
Sophisticated methods are required to determine the functional contribution of circulating cells to fibrosis.
Blocking recruitment factors (e.g., CXCL12, CXCL16) reduced fibrocyte recruitment and subsequent fibrosis.

Conclusions:

Circulating bone marrow-derived cells play a variable but significant role in organ fibrosis.
Identification of recruitment factors provides potential therapeutic targets for managing pathological scarring.
Further research into these mechanisms could lead to novel treatments for fibrotic diseases.