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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
Published on: March 11, 2020
Complete genome sequence of a putative novel victorivirus from Ustilaginoidea virens
Tingting Zhang1, Yinhui Jiang, Junbin Huang
1Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring and Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, 430070 Hubei, China.
Abstract:
Ustilaginoidea virens is the causal agent of a serious disease of rice. Here, we report the presence of five dsRNA bands ranging from about 1.2, 1.5, 1.7, and 1.8 to 5.6 kb in an isolate of this fungus from China and the complete sequence of the largest dsRNA segment, putatively representing the genome of a novel virus, designated as Ustilaginoidea virens RNA virus 1 (UvRV1), UvRV1, which has a genome length of 5567 bp and has two consecutive open reading frames (ORFs) with a five-nucleotide overlap. Phylogenetic analysis showed that UvRV1 belongs to the genus of Victorivirus in the family Totiviridae.
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