Related Experiment Video
Updated: May 14, 2026

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
Published on: June 10, 2009
Virus-aided gene expression and silencing using TRV for functional analysis of floral scent-related genes
Ben Spitzer-Rimon1, Alon Cna'ani, Alexander Vainstein
1The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
Abstract:
Flower scent is a composite character determined by a complex mixture of low-molecular-weight volatile molecules. Despite the importance of floral fragrance, our knowledge on factors regulating these pathways remains sketchy. Virus-induced gene silencing (VIGS) and virus-aided gene expression (VAGE) are characterized by a simple inoculation procedure and rapid results as compared to transgenesis, allowing screening and characterization of scent-related genes. Here, we describe methods using TRV as a VIGS/VAGE vector for the characterization of scent-related genes, protein compartmentalization studies, and protein subcellular targeting.
Related Concept Videos
Experimental RNAi
Reporter Genes
Commonly used reporter...
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

