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Updated: May 14, 2026

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
Determination of monosaccharides derivatized with 2-aminobenzoic Acid by capillary electrophoresis
Mitsuru Abo1, Li-Ping He, Kae Sato
1Yokohama College of Pharmacy, Yokohama, Japan. abo@mail.ecc.u-tokio.ac.jp
Abstract:
Reducing monosaccharides were derivatized with 2-aminobenzoic acid (2-AA) through reductive amination using sodium cyanoborohydride as a reductant, and the derivatives were separated by capillary zone electrophoresis with UV detection using 50 mM sodium phosphate (pH 5.5) or 150 mM sodium borate-50 mM sodium phosphate (pH 7.0) running buffer. The derivatives of monosaccharides, which are major components of various carbohydrate materials, were completely separated within 25 min.

