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Updated: May 14, 2026
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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
Published on: August 21, 2021
Assessment of DNA damage by micellar electrokinetic chromatography
Paolo Fattorini1, Giorgio Marrubini, Pierangela Grignani
1Department of Medicine, surgery and Health, University of Trieste, Trieste, Italy. fattorin@univ.trieste.it
Methods in Molecular Biology (Clifton, N.J.)
|February 7, 2013
Abstract:
A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of uncanonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR templates used in several fields of investigation.
