Comparison of the contribution from different energy-linked reactions to the function of a membrane potential in photosynthetic bacteria
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Updated: May 14, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
B F Nore1, Y Sakai, M Baltscheffsky
1Department of Biochemistry, Arrhenius Laboratories for Natural Science, University of Stockholra, Stockholm Sweden.
Abstract:
The steady-state membrane potentials generated by light, PP(i), ATP or the reverse transhydrogenase reaction were studied in chromatophores from two different phototrophic bacteria, Rhodospirillum rubrum and Rhodopseudomonas viridis. The membrane potentials generated by the different energy-linked reactions were evaluated by a tetraphenylboron(TPB(-)) ion-selective electrode. The generated by light was estimated to be 110 mV and 50 mV in R. rubrum and Rps. viridis chromatophores, respectively. In the dark, PP (i), ATP and reversed transhydrogenase generated membrane potentials in R. rubrum and Rps. viridis chromatophores 50, 60 and 35 mV, and 14, 35 and 25 mV,respectively. The effect of magnesium ion on the membrane potential generated by different energy-linked reactions was also studied. The induced by different energy-generating reactions in R. rubrum and Rps. viridis chromatophores and the possible relationship to the chromatophore structures are discussed.
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