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Updated: May 14, 2026
A Model of Acute Lung Injury Following Visceral Ischemia-Reperfusion by Supra-Coeliac Aortic Cross Clamping in Rats
Published on: August 15, 2025
[IL-6 expression in lung of rats subjected to brain ischemia]
Yan Tan1, Shu-Yi Jiang, Qi-Qin Dan
1Department of Respiration, Affiliated Kunhua Hospital of Kunming Science and Technology University, Kunming 650032, China.
Objective:
To study the expression changes of interleukin-6 (IL-6) in the lung of rats with brain ischemia.
Methods:
Adult SD rats were divided into sham operation group and brain ischemia lung injury (BILI) group randomly. Focal cerebral ischemia inflammatory lung injury model was developed with intraluminal thread technique. RT-PCR was applied to detect the IL-6 expression level of lung at 24 h after brain ischemia. The IL-6 protein in lung at 48 h after brain ischemia was detected with Western blot. Immunohistochemistry method was used to demonstrate the IL-6 protein location in lung at 72 h after brain ischemia.
Results:
Increased immunostaining, protein level and mRNA expression for IL-6 were found in lung at investigated time point after brain ischemia.
Conclusion:
IL-6, as a crucial preinflammatory factor, could be related to airway inflammation in rats induced by brain ischemia.
