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A Model of Acute Lung Injury Following Visceral Ischemia-Reperfusion by Supra-Coeliac Aortic Cross Clamping in Rats
Published on: August 15, 2025
[Expression changes of IL-1beta in lung of rats subjected to brain ischemia]
Qian-Yu Yue1, Qi-Qin Dan, Rong Rong
1Department of Respiration, Affiliated Kunhua Hospital of Kunming Science and Technology University, Kunming 650032, China.
Objective:
To study the expression changes of interleukin-1beta (IL-1beta) in the lung of rats with brain ischemia.
Methods:
Adult SD rats were divided into sham operation group and brain ischemia lung injury (BILI) group randomly. Focal cerebral ischemia inflammatory lung injury model was developed with intraluminal thread technique. Lungs were harvested from rats at different time point respectively. RT-PCR (24 h), Western blot (48 h) and immunohistochemistry (72 h) were employed to detect the expressional changes and the distributions of IL-1beta in the lung tissues.
Results:
IL-1beta immunohistochemical positive reaction was observed in epithelia cell and neutrophil as well as macrophage. Increased protein level and mRNA expression for IL-1beta were found in lung after brain ischemia compared with those of sham group.
Conclusion:
IL-1beta, as a crucial inflammatory factor, could be associated with airway inflammation in lung following brain ischemia in rats.