Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

IR Spectrometers

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

Spectrophotometry: Introduction

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimizing delivery timing of large-for-gestational-age fetuses based on major labor complications (reply to letter to the editor).

American journal of obstetrics and gynecology·2026

Same author

Biology matters in gestational age dating: lessons from assisted reproductive technology pregnancies.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2026

Same author

Routine 36-week scan: optimizing delivery timing of large for gestational age fetuses.

American journal of obstetrics and gynecology·2026

Same author

Soluble biomarkers for immune checkpoint inhibitor-related encephalitis: A mini-review.

Revue neurologique·2024

Same author

An exploration of ecoacoustics and its applications in conservation ecology.

Bio Systems·2024

Same author

Maternal prepregnancy weight as an independent risk factor for congenital heart defect: systematic review and meta-analysis stratified by subtype and severity of defect.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2024

Search research articles

Related Experiment Video

Updated: May 14, 2026

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

Published on: December 22, 2015

Note: Comparison between a prism-based and an acousto-optic tunable filter-based spectrometer for diffusive media.

A Farina¹, I Bargigia, P Taroni

¹Consiglio Nazionale delle Ricerche, Istituto di Fotonica e Nanotecnologie, Piazza L. da Vinci 32, I-20133 Milano, Italy. andrea.farina@polimi.it

The Review of Scientific Instruments

|February 8, 2013

Summary

A dispersive prism system offers superior spectral accuracy for time-resolved spectroscopy compared to an acoustic-optic tunable filter (AOTF). This finding is crucial for accurate analysis of diffusive media, improving absorption and scattering spectra recovery.

More Related Videos

Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces

Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces

Published on: July 26, 2016

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Published on: November 21, 2019

Related Experiment Videos

Last Updated: May 14, 2026

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

Published on: December 22, 2015

Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces

Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces

Published on: July 26, 2016

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Published on: November 21, 2019

Area of Science:

Biomedical Optics
Spectroscopy
Photonics

Background:

Time-resolved spectroscopy is vital for analyzing diffusive media.
Accurate spectral selection is critical for reliable spectroscopic data.
Supercontinuum laser sources offer broad spectral bandwidths for advanced spectroscopic techniques.

Purpose of the Study:

To compare two spectral selection methods for time-resolved spectroscopy of diffusive media.
To evaluate the performance of a dispersive prism versus an acoustic-optic tunable filter (AOTF).
To assess the impact of each system on spectral distortions in recovered absorption and scattering spectra.

Main Methods:

Utilized a supercontinuum laser source for broadband light generation.
Implemented two spectral selection techniques: a dispersive prism and a commercial AOTF.
Performed comparative analysis based on extracted power, spectral response, and spectral distortions.
Validated findings through simulations and experiments on diffusive phantoms.

Main Results:

The acoustic-optic tunable filter (AOTF) exhibited a narrower full width at half maximum (FWHM) bandpass.
The dispersive prism system demonstrated significantly fewer distortions in the retrieved absorption and scattering spectra.
Both methods were evaluated for their effectiveness in time-resolved spectroscopy of diffusive media.

Conclusions:

The dispersive prism approach is superior for spectral selection in time-resolved spectroscopy of diffusive media.
Prism-based systems minimize spectral distortions, leading to more accurate recovery of absorption and scattering properties.
This research provides valuable insights for optimizing spectroscopic instrumentation for biological and material science applications.