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Optimizing Tubulin Yield from Porcine Brain Tissue
Published on: October 11, 2024
Tubulin inhibitors from an endophytic fungus isolated from Cedrus deodara
Manjeet Kumar1, Masroor Qadri, Parduman Raj Sharma
1Natural Product Microbes, Indian Institute of Integrative Medicine, Canal Road, Jammu-Tawi, India.
Abstract:
From an endophytic fungus, a close relative of Talaromyces sp., found in association with Cedrus deodara, four compounds including two new ones (2 and 4) were isolated and characterized. The structures of two compounds (1 and 4) were confirmed by X-ray crystallography. The compounds displayed a range of cytotoxicities against human cancer cell lines (HCT-116, A-549, HEP-1, THP-1, and PC-3). All the compounds were found to induce apoptosis in HL-60 cells, as evidenced by fluorescence and scanning electron microscopy studies. Also, the compounds caused significant microtubule inhibition in HL-60 cells.
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