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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
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Published on: January 31, 2025

VDAC3 and Mps1 negatively regulate ciliogenesis.

Shubhra Majumder1, Harold A Fisk

  • 1Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.

Cell Cycle (Georgetown, Tex.)
|February 8, 2013
PubMed
Summary
This summary is machine-generated.

The voltage-dependent anion channel 3 (VDAC3) protein at the centrosome regulates cilia formation. VDAC3, with Mps1, controls ciliary disassembly during cell cycle entry and suppresses cilia assembly in proliferating cells.

Keywords:
Mps1VDACbasal bodycentrosomeciliogenesisprimary ciliaquiescence

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Area of Science:

  • Cell Biology
  • Mitochondrial Biology
  • Cilia Biology

Background:

  • Centrosomes organize centrioles in cycling cells and primary cilia in quiescent cells.
  • The mitochondrial protein VDAC3 is localized to the centrosome, specifically the mother centriole.
  • VDAC3 influences centriole assembly by recruiting Mps1 to centrosomes.

Purpose of the Study:

  • To investigate the role of VDAC3 in regulating ciliogenesis in both cycling and quiescent cells.
  • To elucidate the mechanism by which VDAC3 controls cilia formation.
  • To explore the potential link between VDAC3, mitochondrial dysfunction, and ciliopathies.

Main Methods:

  • Depletion of VDAC3 in cycling cells.
  • Expression of GFP-VDAC3 in quiescent cells.
  • Investigating the role of Mps1 in VDAC3-mediated ciliogenesis regulation.

Main Results:

  • VDAC3 depletion leads to inappropriate ciliogenesis in cycling cells.
  • GFP-VDAC3 expression suppresses ciliogenesis in quiescent cells.
  • Mps1 negatively regulates ciliogenesis, and its centrosomal recruitment bypasses VDAC3-dependent effects.

Conclusions:

  • A VDAC3-Mps1 module at the centrosome promotes ciliary disassembly during cell cycle entry and suppresses cilia assembly in proliferating cells.
  • VDAC3 plays a critical role in balancing centriole and cilia dynamics.
  • VDAC3 may represent a link between mitochondrial dysfunction and ciliopathies.