Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Interphase00:54

Interphase

The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the cell cycle (G1, S, and G2), and mitosis (M). During interphase, which takes up about 95 percent of the duration of the eukaryotic cell cycle, cells grow and replicate their DNA in preparation for mitosis.
Interphase00:56

Interphase

The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the cell cycle (G1, S, and G2), and mitosis (M). During interphase, which takes up about 95 percent of the duration of the eukaryotic cell cycle, cells grow and replicate their DNA in preparation for mitosis.
Phases of Interphase
Following each period of mitosis and cytokinesis, eukaryotic cells enter interphase, during which they grow and replicate...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LOX, but not LOXL2, promotes bone metastasis formation and bone destruction in triple-negative breast cancer.

Journal of bone oncology·2024
Same author

p27<sup>Kip1</sup> regulates the microtubule bundling activity of PRC1.

Biochimica et biophysica acta. Molecular cell research·2018
Same author

A Conserved Cytoskeletal Signaling Cascade Mediates Neurotoxicity of FTDP-17 Tau Mutations <i>In Vivo</i>.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2017
Same author

Lysyl Oxidase Is a Strong Determinant of Tumor Cell Colonization in Bone.

Cancer research·2016
Same author

WISp39 and Hsp90: actin' together in cell migration.

Oncotarget·2015
Same author

WISp39 binds phosphorylated Coronin 1B to regulate Arp2/3 localization and Cofilin-dependent motility.

The Journal of cell biology·2015
Same journal

ALDH18A1 fuels spermine biosynthesis to sustain ferroptosis resistance in cancer and ischemia-reperfusion injury.

Cell cycle (Georgetown, Tex.)·2026
Same journal

Circular RNA circ_0001829 attenuates G2/M arrest to promote hepatocyte proliferation by sponging miR-3095-3p following liver injury.

Cell cycle (Georgetown, Tex.)·2026
Same journal

Identification of PGF+ endothelial cells associated with plaque instability in carotid atherosclerosis by scRNA-seq and RNA-seq analysis.

Cell cycle (Georgetown, Tex.)·2026
Same journal

BMSCs-derived exosomal miR-196a-5p promotes macrophage M2 polarization and osteogenesis in postmenopausal osteoporosis through regulating Rspo2/Wnt/β-catenin signaling.

Cell cycle (Georgetown, Tex.)·2026
Same journal

MicroRNA-6833-3p drives prostate cancer progression and stemness by targeting the NUMB-mediated NOTCH signaling pathway.

Cell cycle (Georgetown, Tex.)·2026
Same journal

OTUD5 promotes AML progression by stabilizing SLC7A11 to suppress ferroptosis.

Cell cycle (Georgetown, Tex.)·2026
See all related articles

Related Experiment Video

Updated: May 14, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

TD-60 is required for interphase cell cycle progression.

Mythili Yenjerla1, Andreas Panopoulos, Caroline Reynaud

  • 1Tumor Development Program, Sanford-Burnham Medical Research Institute, La Jolla, CA, USA.

Cell Cycle (Georgetown, Tex.)
|February 8, 2013
PubMed
Summary
This summary is machine-generated.

TD-60 (RCC2) is crucial for cell cycle progression in both interphase and mitosis. This essential protein regulates G1/S and G2/M transitions, impacting cell division and potentially "moonlighting" with distinct functions.

Keywords:
G1 phaseG2 phaseRCC2checkpointmicrotubules

More Related Videos

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

Use of Drosophila S2 Cells for Live Imaging of Cell Division
06:17

Use of Drosophila S2 Cells for Live Imaging of Cell Division

Published on: August 23, 2019

Related Experiment Videos

Last Updated: May 14, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

Use of Drosophila S2 Cells for Live Imaging of Cell Division
06:17

Use of Drosophila S2 Cells for Live Imaging of Cell Division

Published on: August 23, 2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • TD-60 (RCC2) was previously identified as a mitotic centromere-associated protein essential for mitosis completion.
  • Its role in interphase cell cycle regulation was previously unknown.

Purpose of the Study:

  • To investigate the function of TD-60 (RCC2) during interphase cell cycle progression.
  • To elucidate the molecular mechanisms underlying TD-60's dual roles in interphase and mitosis.

Main Methods:

  • RNA interference (siRNA) to suppress TD-60 expression in mammalian cells.
  • Analysis of cell cycle progression at G1/S and G2/M checkpoints.
  • Co-immunoprecipitation assays to identify interacting proteins.

Main Results:

  • siRNA suppression of TD-60 caused cell cycle arrest at G1/S and G2/M phases in both normal and p53-deficient cells.
  • TD-60 interacts with Rac1 and Arf6, and is part of the α5β1 integrin and cortactin interactomes.
  • TD-60 is required for proper mitotic spindle assembly and mitotic protein activation during mitosis.

Conclusions:

  • TD-60 (RCC2) is an essential regulator of cell cycle progression during both interphase and mitosis.
  • TD-60 likely functions through distinct mechanisms in interphase and mitosis, exhibiting a "moonlight" phenomenon.
  • These findings highlight TD-60 as a critical component in cell cycle control signaling pathways.