Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On the mechanism and voltage sensitivity of voltage-sensitive second harmonic probes: A case study of FM4-64.

Biophysical journal·2026
Same author

Second harmonic imaging of membrane potentials: Comparing water and chromophores as probes.

Biophysical journal·2026
Same author

Encapsulating Textiles with Dynamic Covalent Networks for Sustainable and Efficient Oil Spill Cleanup.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Characterizing programmed cell death features in osteoarthritis through integrative multiomics and machine learning analysis.

PeerJ·2025
Same author

Unraveling the Molecular Pathways for Structure "Making" and "Breaking" by Ions in Water.

Journal of the American Chemical Society·2025
Same author

Interfacial Self-Assembly of Sugars at Nanoscale Membranes Leads to Micron-Scale, Spectroscopically Ice-Like Chiral Suprastructures of Water.

Journal of the American Chemical Society·2025

Related Experiment Video

Updated: May 14, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Label-free second harmonic and hyper Rayleigh scattering with high efficiency.

Nikolaos Gomopoulos1, Cornelis Lütgebaucks, Qinchao Sun

  • 1Institute of Bio-engineering (IBI), School of Engineering (STI), Ecole Polytechnique Federale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

Optics Express
|February 8, 2013
PubMed
Summary

We developed a label-free method using hyper Rayleigh scattering to study transport in biological systems. This technique enables millisecond-timescale measurements of aqueous solutions, liposomes, and nanoparticles.

More Related Videos

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators
12:21

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators

Published on: April 4, 2016

Related Experiment Videos

Last Updated: May 14, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators
12:21

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators

Published on: April 4, 2016

Area of Science:

  • Nonlinear optics
  • Biophysics
  • Materials science

Background:

  • Label-free optical techniques are crucial for studying dynamic biological processes.
  • Current methods often lack the speed or sensitivity for real-time interfacial measurements.
  • Understanding transport across membranes and through nanoparticle interfaces is key in biology and materials science.

Purpose of the Study:

  • To develop a rapid, label-free method for analyzing interfacial and aqueous solution dynamics.
  • To enable time-resolved measurements of transport phenomena in biological and synthetic systems.
  • To demonstrate the application of hyper Rayleigh scattering and second harmonic scattering for unlabeled samples.

Main Methods:

  • Utilized hyper Rayleigh scattering (HRS) for aqueous solutions.
  • Employed second harmonic scattering (SHS) for unlabeled interfaces of liposomes and nanoparticles.
  • Achieved measurements on a millisecond timescale.

Main Results:

  • Successfully performed HRS from aqueous solutions.
  • Successfully performed SHS from unlabeled liposome and nanoparticle interfaces.
  • Demonstrated millisecond-timescale resolution for interfacial and aqueous responses.

Conclusions:

  • The developed method provides a powerful tool for label-free, time-resolved analysis of transport processes.
  • This technique opens new avenues for studying dynamic interfacial phenomena in biological membranes and nanomaterials.
  • Future applications include real-time monitoring of drug delivery and membrane permeability.