Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Computational and experimental investigation of nanoparticle effects on tissue optical properties and optical coherence tomography imaging.

Biomedical optics express·2026
Same author

Spectral Studies of a Novel Photosensitizer Complex for Photodynamic Therapy.

Current pharmaceutical biotechnology·2026
Same author

Metal-Enhanced Fluorescence of Nanocomplexes.

Materials (Basel, Switzerland)·2026
Same author

Optical Coherence Tomography Mapping of the Scattering Coefficient to Study the Skin Hydration Process under Optical Clearing.

Skin pharmacology and physiology·2026
Same author

Biophysical Alterations of the Stratum Corneum Induced by Optical Clearing Agents: Implications for Efficiency and Safety Optimization.

Skin pharmacology and physiology·2026
Same author

Next-generation biomedical sensing through photoacoustic-electrochemical synergy.

Medical review (2021)·2026

Related Experiment Video

Updated: May 14, 2026

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

Blood optical clearing studied by optical coherence tomography.

Olga Zhernovaya1, Valery V Tuchin, Martin J Leahy

  • 1University of Limerick, Department of Physics and Energy, National Biophotonics and Imaging Platform, Tissue Optics and Microcirculation Imaging Facility, Ireland, Ireland. olga.zhernovaya@ul.ie

Journal of Biomedical Optics
|February 8, 2013
PubMed
Summary

Optical clearing agents improve light penetration depth in blood, enhancing imaging quality for techniques like optical coherence tomography (OCT). This research investigates various agents to reduce light scattering in biological tissues.

More Related Videos

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

Related Experiment Videos

Last Updated: May 14, 2026

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

Area of Science:

  • Biomedical Optics
  • Biophotonics
  • Medical Imaging

Background:

  • Light scattering in biological tissues limits optical imaging depth and quality.
  • Optical clearing methods offer a solution by reducing light scattering.
  • Techniques like optical coherence tomography (OCT) benefit from improved imaging depth.

Purpose of the Study:

  • To investigate the efficacy of optical clearing agents in enhancing light penetration in blood.
  • To evaluate the impact of different clearing agents on light transport in biological tissues.
  • To assess the application of optical clearing in conjunction with OCT for improved imaging.

Main Methods:

  • Utilized an optical coherence tomography (OCT) system to measure light penetration depth.
  • Applied various biocompatible clearing agents, including polyethylene glycol, polypropylene glycol, and propylene glycol solutions.
  • Investigated the influence of these agents on light transport in blood within a mouse tail vein model.

Main Results:

  • Demonstrated improved light penetration depth in blood after the application of specific optical clearing agents.
  • Quantified the reduction in light scattering and enhancement in transmittance.
  • Observed the effects of clearing agents on light transport dynamics in vascular tissue.

Conclusions:

  • Optical clearing agents significantly enhance light penetration in blood, improving OCT imaging capabilities.
  • The choice of clearing agent influences the extent of light scattering reduction and imaging depth.
  • This approach holds promise for advancing in vivo optical imaging of biological tissues.