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Related Experiment Video

Updated: May 14, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

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A c-Myc-MicroRNA functional feedback loop affects hepatocarcinogenesis.

Han Han¹, Dan Sun, Wenjuan Li

¹College of Life Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan, China.

Hepatology (Baltimore, Md.)

|February 8, 2013

Summary

The c-Myc (Myc) oncogene drives liver cancer by disrupting a feedback loop involving miR-148a-5p and miR-363-3p. These microRNAs normally suppress Myc, but their dysregulation promotes hepatocellular carcinoma (HCC) development.

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Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Area of Science:

Hepatology
Molecular Biology
Oncology

Background:

c-Myc (Myc) is crucial for liver development and tumorigenesis.
Pathological activation of Myc is essential for hepatocellular carcinoma (HCC) progression.

Purpose of the Study:

To elucidate the novel microRNA (miRNA)-mediated feedback loop involving Myc in HCC.
To define the roles of miR-148a-5p and miR-363-3p as regulators of Myc in hepatocarcinogenesis.

Main Methods:

Investigated Myc's interaction with miR-148a-5p, miR-363-3p, and USP28.
Analyzed miRNA promoter binding and target interactions.
Assessed the impact of miRNA inhibition/activation on hepatocellular tumorigenesis and cell cycle progression.

Main Results:

Myc directly represses miR-148a-5p and miR-363-3p expression.
miR-148a-5p inhibits Myc, while miR-363-3p destabilizes Myc via USP28 inhibition.
Inhibition of these miRNAs promotes HCC by advancing the G1 to S phase transition.

Conclusions:

miR-148a-5p and miR-363-3p act as negative regulators of Myc.
These miRNAs play significant, previously unrecognized roles in preventing hepatocarcinogenesis.