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Determination of sperm acrosin activity in the arctic fox (Alopex lagopus L.)--using method developed for human
K Stasiak1, B Janicki, J Glogowski
1University of Technology and Life Sciences in Bydgoszcz, Faculty of Animal Breeding and Biology, Department of Biology of Small Ruminants and Environmental Biochemistry, Mazowiecka 28, 85-084 Bydgoszcz, Poland. stasiak@utp.edu.pl
Abstract:
The aim of the study was to adapt a method to determine acrosin activity of human spermatozoa to arctic fox (Alopex lagopus L.) spermatozoa. We modified this method by reducing sperm count per sample from 1 divided by 10 x 10(6) to 25 divided by 200 x 10(3), incubation time from 180 minutes to 60 minutes, and Triton X-100 concentration in the reaction mixture from 0.01% to 0.005% per 100 cm3. It has also confirmed that arctic fox seminal plasma is rich in proteinases and their inhibitors. To completely abolish the inhibitory effect of seminal plasma on acrosin activity it is recommended to wash the spermatozoa four times. Benzamidine served an inhibitor of acrosin activity.
