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Updated: May 14, 2026

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Published on: October 11, 2022
A yeast telomerase complex containing the Est1 recruitment protein is assembled early in the cell cycle
Timothy M Tucey1, Victoria Lundblad
1Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Abstract:
In budding yeast, association of the Est1 regulatory protein with telomerase is thought to be limited to the late S phase, when telomere elongation occurs. By monitoring the stoichiometry of telomerase subunits, we show instead that a telomerase complex containing Est1 is assembled much earlier in the cell cycle. We also report a biochemical interaction between Est1 and the telomere binding protein Cdc13 that recapitulates the previously observed genetic relationship between EST1 and CDC13. This supports a model in which regulated binding of Cdc13 to chromosome termini dictates subsequent interaction of a recruitment-competent telomerase complex with telomeres.
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