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Updated: May 14, 2026
Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
Published on: November 8, 2018
Improvised microinjection technique for mosquito vectors
S Sampath Kumar1, H P Puttaraju
1Department of Sericulture & Division of Biological Sciences, School of Natural Sciences, Bangalore University, Bangalore, India.
Background & Objectives:
Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection.
Methods:
Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult.
Results:
The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability.
Interpretation & Conclusions:
The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.
