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Measurement of barley β-glucan concentration in the plasma by sandwich ELISA using rat dectin-1
Shingo Hino1, Hikaru Sato, Tsukasa Matsuda
1Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Ohya, Shizuoka, Japan.
Bioscience, Biotechnology, and Biochemistry
|February 9, 2013
Abstract:
We used recombinant rat dectin-1 proteins to newly establish sandwich ELISA for determining barley β-glucan (BβG). The ELISA method had a working range of 15-4,000 µg/L. Plasma BβG was detectable up to 24 h after an intravenous administration of BβG (1 mg/kg of body weight) to rats. This method may be an effective tool for investigating the immune modulatory effects of BβG.
