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Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

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Related Experiment Video

Updated: May 14, 2026

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Published on: July 11, 2019

Fluorescence imaging in the last two decades.

Atsushi Miyawaki¹

¹Brain Science Institute, RIKEN and Life Function and Dynamics, ERATO, JST, 2-1 Hirosawa, Wako-city, Saitama, Japan. matsushi@brain.riken.jp

Microscopy (Oxford, England)

|February 9, 2013

Summary

Green fluorescent protein (GFP) imaging has revolutionized neuroscience over 20 years. Advanced techniques like optogenetics and 3D reconstruction now enable unprecedented cellular-level biological insights.

Area of Science:

Molecular Biology
Neuroscience
Biotechnology
Microscopy

Background:

The 20th anniversary of green fluorescent protein (GFP) gene cloning marks significant advancements in fluorescence imaging.
The diversification of fluorescence imaging technologies presents challenges for comprehensive reviews.
Focus is placed on optogenetics and large-scale 3D reconstruction as key innovations.

Purpose of the Study:

To reflect on the development of fluorescence imaging technology over the past two decades.
To highlight the impact of optogenetics and 3D reconstruction in neuroscience.
To illustrate improved understanding of cellular-level biological functions using modern imaging.

Main Methods:

Review of advancements in fluorescence imaging, specifically optogenetics and large-scale 3D reconstruction.

More Related Videos

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

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Last Updated: May 14, 2026

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Published on: July 11, 2019

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Integration of molecular biology and light microscopy techniques.

Application of imaging approaches to study biomolecule transport across the nuclear membrane.

Main Results:

Optogenetics and large-scale 3D reconstruction have emerged as powerful tools in neuroscience.
Modern fluorescence imaging enhances understanding of spatiotemporal regulation of biological functions.
Specific findings on biomolecule movement across the nuclear membrane are presented as an example.

Conclusions:

Fluorescence imaging technologies have dramatically advanced over the last 20 years.
These innovations, driven by interdisciplinary collaboration, provide unprecedented insights into cellular processes.
Techniques now possible were inconceivable two decades ago, transforming biological research.