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Updated: May 14, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
Impact of global and gene-specific DNA methylation pattern in relapsed multiple myeloma patients treated with
Carlos Fernández de Larrea1, Beatriz Martín-Antonio, Maria Teresa Cibeira
1Amyloidosis and Myeloma Unit, Department of Hematology, Hospital Clínic, Barcelona, Institut d'Investigacions Biomèdiques August Pi I Sunyer, José Carreras Leukaemia Research Institute, University of Barcelona, Barcelona, Spain.
Abstract:
We studied seventy-five patients with relapsed MM treated with bortezomib-based regimens. DNA was isolated from bone marrow samples at the time of relapse. Global methylation was determined by ELISA, and CpG island DNA methylation profile of 30 genes by a DNA methylation PCR system. Patients with more than 3.95% of total DNA methylated achieved better overall survival (OS) (p=0.004). A relatively low methylation percentage (<1.07%) of NFKB1 was also associated with longer OS after bortezomib treatment (p=0.015). The combination of highly methylated global genome with low NFKB1 methylation status defined a specific subset of patients with better prognosis.
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