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Updated: May 14, 2026
08:54
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
Robust, synergistic regulation of human gene expression using TALE activators
Morgan L Maeder1, Samantha J Linder, Deepak Reyon
1Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA.
Nature Methods
|February 12, 2013
Summary
Transcription activator-like effector (TALE) activators can effectively boost human gene expression, individually or combined. This research enhances TALE activator utility for research and therapeutic applications.
Area of Science:
- Molecular Biology
- Synthetic Biology
- Gene Regulation
Background:
- Transcription activator-like effector (TALE) technology enables artificial activator design.
- Previous studies indicate monomeric TALE activators often have limited activity.
Purpose of the Study:
- To investigate the functional capacity of TALE activators in modulating endogenous human gene expression.
- To explore the synergistic potential of TALE activators in combination.
Main Methods:
- Utilized TALE activator technology to target and modulate endogenous human genes.
- Assessed gene expression levels across a range of TALE activator designs and combinations.
Main Results:
- Demonstrated robust individual and synergistic function of TALE activators.
- Showcased the ability to increase endogenous gene expression over wide dynamic ranges.
- Validated TALE activators as potent tools for gene expression modulation.
Conclusions:
- TALE activators exhibit significant potential for both research and therapeutic applications.
- Findings guide the design of more effective monomeric TALE-based fusion proteins.
- This work broadens the utility of TALE technology in synthetic biology and gene therapy.
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