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Updated: May 14, 2026

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
10:14

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Published on: December 17, 2013

Quantitating lymphocyte programmed cell death in vitro using simple kill assays.

Lixin Zheng1

  • 1Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. lzheng@niaid.nih.gov

Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2013
PubMed
Summary

Programmed cell death (PCD) is vital for immune response regulation. This study presents two simple in vitro methods to measure lymphocyte PCD by detecting changes in cell membranes and mitochondrial potential.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Lymphocyte homeostasis is crucial for immune response contraction.
  • Activated lymphocytes are prone to programmed cell death (PCD) from various stimuli.
  • Assessing lymphocyte PCD is essential for understanding immune regulation.

Purpose of the Study:

  • To outline simple, versatile methods for measuring lymphocyte PCD in vitro.
  • To provide techniques applicable across different PCD-inducing stimuli.
  • To establish foundational methods for further PCD research.

Main Methods:

  • Culture of lymphocytes in vitro.
  • Assessment of plasma membrane integrity.
  • Measurement of mitochondrial membrane potential changes.
  • Utilizing techniques applicable regardless of the PCD stimulus.

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Last Updated: May 14, 2026

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
10:14

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Published on: December 17, 2013

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
09:28

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

Real-time Cytotoxicity Assays in Human Whole Blood
08:27

Real-time Cytotoxicity Assays in Human Whole Blood

Published on: November 7, 2014

Main Results:

  • Demonstrated two straightforward approaches for quantifying lymphocyte PCD.
  • Showcased methods detecting characteristic PCD-related cellular changes.
  • Validated techniques for in vitro lymphocyte cell death assessment.

Conclusions:

  • The presented methods offer a simple and broadly applicable means to measure lymphocyte PCD.
  • These techniques are valuable for studying immune response contraction and lymphocyte homeostasis.
  • The methods serve as a basis for more detailed PCD investigations.