Related Experiment Video
Updated: May 14, 2026
Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
Published on: July 22, 2011
In vitro suppression assay for functional assessment of human regulatory T cells
1Division of Pediatric Research Center, Department of Pediatrics, The University of Texas Medical School at Houston, Houston, TX, USA. dat.q.tran@uth.tmc.edu
FOXP3(+) regulatory T cells (Tregs) play an important role in controlling immune activation, maintenance of -homeostasis and the prevention of autoimmunity. Much effort has been focused in assessing their potential defects in certain human diseases and in developing potential Treg immunotherapy to cure autoimmune conditions. One cardinal feature of Tregs is their ability to suppress the activation and proliferation of effector T cells. The in vitro suppression assay is a convenient and relatively reliable method to quantify their suppressive function and validate their identity as true Tregs. This protocol describes a reliable, nonradioactive in vitro suppression assay to assess the immunosuppressive property of human Tregs in inhibiting proliferation of dye-labeled responder T cells using non-irradiated, HLA-DR(+) antigen presenting cells and soluble anti-CD3 antibodies as stimuli.
FOXP3(+) regulatory T cells (Tregs) play an important role in controlling immune activation, maintenance of -homeostasis and the prevention of autoimmunity. Much effort has been focused in assessing their potential defects in certain human diseases and in developing potential Treg immunotherapy to cure autoimmune conditions. One cardinal feature of Tregs is their ability to suppress the activation and proliferation of effector T cells. The in vitro suppression assay is a convenient and relatively reliable method to quantify their suppressive function and validate their identity as true Tregs. This protocol describes a reliable, nonradioactive in vitro suppression assay to assess the immunosuppressive property of human Tregs in inhibiting proliferation of dye-labeled responder T cells using non-irradiated, HLA-DR(+) antigen presenting cells and soluble anti-CD3 antibodies as stimuli.
More Related Videos
08:20In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Published on: December 30, 2016
18:48In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
Published on: August 12, 2017