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Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
Published on: October 6, 2022
Upregulated H19 contributes to bladder cancer cell proliferation by regulating ID2 expression
Ming Luo1, Zuowei Li, Wei Wang
1Department of Urology, Affiliated Tenth People's Hospital, Tongji University, Shanghai, China.
The FEBS Journal
|February 13, 2013
Summary
Upregulated long noncoding RNA 19 (H19) promotes bladder cancer cell proliferation by increasing Inhibitor of DNA binding/differentiation 2 (ID2) expression. This finding offers potential therapeutic targets for bladder cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long noncoding RNAs (lncRNAs) play crucial regulatory roles in cancer biology.
- The lncRNA H19 (H19) is essential for human tumor growth, but its specific role in bladder cancer proliferation is not well understood.
Purpose of the Study:
- To investigate the expression and function of H19 in bladder cancer.
- To elucidate the relationship between H19 and Inhibitor of DNA binding/differentiation 2 (ID2) in bladder cancer progression.
Main Methods:
- Real-time PCR was used to evaluate H19 and ID2 expression levels in bladder cancer tissues and cells.
- Gain-of-function and loss-of-function studies were performed to assess the impact of H19 on cell proliferation and ID2 expression.
- Correlation analysis was conducted to determine the relationship between H19 and ID2 levels.
Main Results:
- H19 expression was significantly increased in bladder cancer tissues compared to normal controls.
- Forced expression of H19 promoted bladder cancer cell proliferation in vitro.
- ID2 expression was upregulated in bladder cancer, and its levels positively correlated with H19 levels.
- Overexpression of H19 increased ID2 expression, while H19 knockdown decreased it.
- Upregulated H19 enhanced bladder cancer cell proliferation by increasing ID2 expression.
Conclusions:
- Upregulated H19 contributes to bladder cancer growth by regulating ID2 expression.
- H19 and ID2 may serve as potential therapeutic targets for bladder cancer treatment.
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