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Updated: May 14, 2026

Real-Time Force Measurement Between Emulsion Droplets During Enzymatic Breakdown
Published on: June 27, 2025
Influence of environmental stresses on the stability of W/O/W emulsions containing enzymatically modified starch
Saehun Mun1, Yongdoo Choi, Kwan-Hwa Park
1Center for Food and Bioconvergence and Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
Abstract:
The present study was performed to investigate the stability of W/O/W emulsions containing 4-α-glucanotransferase (4αGTase)-treated starch against environmental stresses such as heating, shearing, and repeated freeze-thawing. W/O/W emulsions were subjected to thermal processing at different temperatures ranging from 30 to 90 °C for 30 min, constant shear for 0-7 min, and freeze-thaw cycling between -20 °C and 30 °C, respectively, and followed by encapsulation efficiency (EE) measurement. As for the case of thermal stress, it was clearly shown that addition of 4αGTase-treated starch in the internal aqueous phase of emulsions helped to maintain higher EE during thermal processing. However, at lower PGPR level (2%), the addition of 4αGTase-treated starch dramatically reduced EE at temperatures higher than 70 °C, which was probably related to the melting of 4αGTase-treated starch gel. The incorporation of 4αGTase-treated starch improved the stability of emulsions during shearing process, but could not prevent W/O/W emulsions from creaming and destabilizing during freeze-thaw cycling.
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