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Related Experiment Videos

A glycogen precursor associated with membrane in retina.

E R Lacoste1, M C Miozzo, J A Curtino

  • 1Departamento de Quimica Biologica-CIQUIBIC, Facultad de Ciencias Quimicas-CONICET, Universidad Nacional de Cordoba, Argentina.

The Biochemical Journal
|May 1, 1990
PubMed
Summary

Retina glycogen synthesis begins on membrane-associated protein p42, forming insoluble proteoglycogen. This is then released and converted to soluble proteoglycogen through polysaccharide growth.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Retinal Research

Background:

  • Proteoglycogen is a complex of protein and glycogen.
  • Retinal microsomes contain proteoglycogen fractions.

Purpose of the Study:

  • To investigate the biogenesis of proteoglycogen in retinal microsomes.
  • To elucidate the relationship between acid-insoluble and acid-soluble proteoglycogen fractions.

Main Methods:

  • Incorporation of [14C]glucose from UDP-[14C]glucose into proteoglycogen.
  • Analysis of labelling rates at varying sugar-donor concentrations.
  • Chase experiments using unlabelled UDP-glucose.
  • Size exclusion chromatography (Sephacryl S-500).
  • Differential extraction with saline, SDS, and detergents.

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Main Results:

  • Acid-insoluble proteoglycogen (Mr 4.7 x 10^5) serves as a precursor to acid-soluble proteoglycogen (Mr 7.0 x 10^5).
  • A significant portion of acid-insoluble proteoglycogen is associated with retinal membrane vesicles.
  • Proteoglycogen is tightly bound to membranes, requiring specific detergents for dissociation.
  • Detergent treatment conditions also solubilize integral membrane sialoglycoconjugates.

Conclusions:

  • Glycogen biogenesis in the retina initiates on membrane-associated protein p42, forming acid-insoluble proteoglycogen.
  • This insoluble form is subsequently released from membranes and transformed into the acid-soluble form via polysaccharide elongation.