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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

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Related Experiment Video

Updated: May 14, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Alternative splicing switching in stem cell lineages.

Iouri Chepelev1, Xin Chen

  • 1Systems Biology Center, National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892, USA.

Frontiers in Biology
|February 13, 2013
PubMed
Summary

Stem cell pluripotency and differentiation involve specific mRNA isoforms. Alternative splicing patterns are crucial for maintaining stem cell identity and regulating reprogramming into induced pluripotent stem cells (iPSCs).

Keywords:
adult stem cellsalternative splicingembryonic stem cellsepigenetic regulationpost-transcriptional regulationstem cell maintenance and differentiation

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Last Updated: May 14, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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Published on: April 26, 2017

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Published on: April 22, 2021

Area of Science:

  • Stem cell biology
  • Molecular mechanisms of pluripotency
  • Regenerative medicine

Background:

  • Stem cell pluripotency is key to regenerative medicine.
  • Transcription factors and chromatin regulate stem cell identity.
  • Alternative splicing represents a distinct molecular feature of stem cells.

Purpose of the Study:

  • To review the role of mRNA isoform switching in stem cell pluripotency.
  • To discuss the interaction of splicing with other molecular mechanisms.
  • To explore splicing's role in induced pluripotent stem cell (iPSC) reprogramming.

Main Methods:

  • Literature review of studies on stem cell molecular mechanisms.
  • Analysis of research on alternative splicing patterns in stem cells.
  • Examination of data on transcriptional networks and chromatin landscapes.

Main Results:

  • Stem cell-specific mRNA isoforms likely maintain cell identity and activity.
  • Alternative splicing patterns are distinct in stem cells.
  • Splicing changes are implicated in differentiation and iPSC reprogramming.

Conclusions:

  • Alternative mRNA splicing is a significant regulator of stem cell pluripotency.
  • Splicing interacts with other molecular pathways to control stem cell fate.
  • Understanding splicing is vital for advancing regenerative medicine and iPSC technology.