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Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity
Published on: May 6, 2019
Lens aberrations and their relationship with lens sutures for species with Y-suture branches
Ana Gargallo1, Justo Arines, Eva Acosta
1Universidade de Santiago de Compostela, Departamento de Física Aplicada (área de Óptica), Facultade de Física (Campus Vida), 15782 Santiago de Compostela, Spain. ana.gargallo@usc.es
Abstract:
Work remains to be done to understand the origins of ocular aberrations. We analyze lens aberrations of several species with Y-suture branches (bovine, ovine, and porcine) and their relationship with suture distribution. Aberrations are measured in vitro with a point diffraction interferometer in 10 different eyes of each species. The minimum number of Zernike polynomials minimizing the root mean square error of the wavefront is estimated by processing the interferograms. Through this we find significant amounts of astigmatism, coma, spherical aberration, and trefoil in the lenses of the three species. Moreover, we observe a high degree of correlation between the orientation of the lens sutures and the axis of nonrotationally symmetric aberrations. Our results point to lens sutures as the histological origin of the most significant lens aberrations: astigmatism, coma, and trefoil, but we are unable to find a major suture governing all the axes.
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