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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

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Circulating microRNA expression profiles associated with systemic lupus erythematosus.

Anting Liu Carlsen1, Aaron J Schetter, Christoffer T Nielsen

  • 1Statens Serum Institut, Copenhagen, Denmark.

Arthritis and Rheumatism
|February 13, 2013
PubMed
Summary

Specific circulating microRNAs (miRNAs) are altered in systemic lupus erythematosus (SLE). A four-miRNA signature accurately diagnoses SLE and distinguishes it from other autoimmune diseases, offering insights into disease pathways.

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Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
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Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Published on: April 29, 2022

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Last Updated: May 14, 2026

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
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Published on: June 13, 2016

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
06:48

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Published on: April 29, 2022

Area of Science:

  • Immunology
  • Genetics
  • Molecular Biology

Background:

  • Systemic lupus erythematosus (SLE) is a complex autoimmune disease with diverse clinical manifestations.
  • Identifying specific biomarkers for SLE diagnosis and monitoring is crucial for effective management.

Purpose of the Study:

  • To investigate the diagnostic specificity of cell-free circulating microRNAs (miRNAs) in patients with SLE.
  • To identify miRNA expression patterns associated with SLE activity, particularly nephritis.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to analyze miRNA expression in plasma from 409 individuals.
  • The study included patients with SLE, healthy controls, and patients with other autoimmune diseases (vasculitis, rheumatoid arthritis).
  • Validation was performed in independent cohorts to confirm the diagnostic accuracy of identified miRNA signatures.

Main Results:

  • Seven miRNAs showed statistically significant differential expression in SLE patients.
  • A four-miRNA signature demonstrated high accuracy (76%) in differentiating SLE patients from controls.
  • Specific miRNA profiles were associated with active nephritis in SLE patients.
  • The four-miRNA model effectively distinguished SLE from most other autoimmune conditions, except vasculitis.

Conclusions:

  • Circulating miRNA patterns are systematically altered in SLE, serving as potential diagnostic biomarkers.
  • A four-miRNA signature is diagnostic for SLE and can differentiate it from other inflammatory conditions.
  • Identified miRNAs target key pathways involved in SLE pathogenesis, including TGF-β signaling, apoptosis, and immune cell regulation.