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Neural responses to electrical stimulation on patterned silk films.

Marie Hronik-Tupaj1, Waseem Khan Raja, Min Tang-Schomer

  • 1Department of Biomedical Engineering, Science & Technology Center, Tufts University, Medford, Massachusetts 02155, USA.

Journal of Biomedical Materials Research. Part A
|February 13, 2013
PubMed
Summary

Surface patterning and electrical stimulation significantly improve nerve axon regeneration and alignment after injury. This combined approach offers enhanced control over nerve axon outgrowth and guidance for functional recovery.

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Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Regenerative Medicine

Background:

  • Peripheral nerve injuries (PNI) are a significant cause of sensory and motor function loss.
  • Incomplete or misdirected axon regeneration after PNI leads to functional deficits.
  • Developing strategies to promote controlled axon regeneration is crucial for patient recovery.

Purpose of the Study:

  • To investigate the effects of surface patterning and electrical stimulation on peripheral nerve axon outgrowth and alignment.
  • To assess the potential of patterned electronic silk films to guide neuronal regeneration.
  • To compare the efficacy of combined surface patterning and electrical stimulation against individual or absent stimuli.

Main Methods:

  • Sputter coating of metal electrodes onto micropatterned silk protein films with specific groove dimensions (3.5 μm wide × 500 nm deep).
Keywords:
biomaterialelectrical stimulationneuronsilktissue engineering

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  • Seeding of P19 neurons onto patterned and flat electronic silk films.
  • Application of electrical stimulation (120 mV, 1 kHz, 45 min daily for 7 days) to specific groups.
  • Comparative analysis of axon outgrowth and alignment across different experimental conditions.

    • Main Results:

    • Significant axon alignment was observed on patterned silk films compared to flat films.
    • Axon outgrowth was significantly greater on electronic films on days 5 and 7 compared to unstimulated groups.
    • The combination of surface patterning and electrical stimulation demonstrated enhanced control over nerve axon regeneration.

    Conclusions:

    • Surface patterning of silk protein films effectively promotes axon alignment.
    • Electrical stimulation, when applied to patterned electronic films, significantly enhances axon outgrowth.
    • The combined approach of surface patterning and electrical stimulation offers a promising strategy for controlling nerve axon regeneration and alignment after injury.