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Direct DNA sequencing of PCR amplified genomic DNA by the Maxam-Gilbert method
T Tahara1, J P Kraus, L E Rosenberg
1Yale University School of Medicine, Department of Human Genetics, New Haven, CT 06510.
Biotechniques
|April 1, 1990
Abstract:
We present a method to directly sequence PCR amplification products utilizing the chemical method of Maxam and Gilbert. This procedure yields clearly readable DNA sequences of 100-400 base pairs in length derived from human genomic DNA in four days' time.