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Related Experiment Video

Updated: May 14, 2026

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization
03:08

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization

Published on: October 3, 2025

Protein target quantification decision tree.

Jong Won Kim1, Jinsam You

  • 1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, MS 4053, Indianapolis, IN 46202, USA.

International Journal of Proteomics
|February 13, 2013
PubMed
Summary
This summary is machine-generated.

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Decision Making: P-value Method01:09

Decision Making: P-value Method

The process of hypothesis testing based on the P-value method includes calculating the P- value using the sample data and interpreting it.
First, a specific claim about the population parameter is proposed. The claim is based on the research question and is stated in a simple form. Further, an opposing statement to the claim  is also stated. These statements can act as null and alternative hypotheses:  a null hypothesis would be a neutral statement while the alternative hypothesis can have a...

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Mass spectrometry (MS) is crucial for identifying disease biomarkers. New targeted proteomics methods enhance validation, paving the way for clinical diagnostics and treatments.

Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Clinical Mass Spectrometry

Background:

  • Mass spectrometry (MS)-based proteomic platforms are increasingly utilized in research.
  • MS offers high selectivity and sensitivity for identifying potential disease biomarkers.
  • Lack of validation hinders the clinical approval of identified biomarker candidates by regulatory bodies like the FDA.

Purpose of the Study:

  • To review recent advancements in mass spectrometry technology.
  • To discuss the application of targeted proteomics methods for biomarker validation.
  • To highlight the importance of robust validation for clinical translation of biomarkers.

Main Methods:

  • Review of recent developments in mass spectrometry instrumentation.
  • Discussion of targeted proteomics strategies for protein biomarker validation.

Related Experiment Videos

Last Updated: May 14, 2026

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization
03:08

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization

Published on: October 3, 2025

  • Analysis of challenges and opportunities in biomarker verification.

    • Main Results:

    • Modern mass spectrometers offer enhanced sensitivity, resolution, and mass accuracy.
    • Targeted proteomics approaches significantly improve the capabilities for protein biomarker validation.
    • The potential for developing clinically applicable biomarkers is increased through advanced validation techniques.

    Conclusions:

    • Successful validation of protein biomarkers is essential for improved disease diagnostics, prognostics, and treatment strategies.
    • Recent technological improvements in MS and targeted proteomics facilitate biomarker validation.
    • This review underscores the critical role of validation in translating proteomic discoveries into clinical practice.