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Related Experiment Video

Updated: May 14, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

Fast and specific dermatophyte detection by automated DNA extraction and real-time PCR.

A Bergman¹, D Heimer, N Kondori

¹Department of Clinical Microbiology, Unilabs AB, Skövde, Sweden. anna.bergman@unilabs.com

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases

|February 14, 2013

Summary

A new real-time PCR method accurately detects dermatophytes, including Trichophyton rubrum and Trichophyton interdigitale, in clinical samples. This fungal DNA test is more sensitive and significantly faster than traditional culture methods.

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Area of Science:

Medical Mycology
Molecular Diagnostics

Background:

Dermatophytosis diagnosis relies on culture, which is slow.
Rapid and sensitive detection methods are needed for effective treatment.

Purpose of the Study:

Develop and validate a real-time PCR assay for dermatophyte detection.
Identify specific species like Trichophyton rubrum and Trichophyton interdigitale.

Main Methods:

Direct DNA extraction from clinical samples using automated MagNA Pure Compact.
Real-time PCR assay targeting dermatophyte species.
Comparison with conventional fungal culture.

Main Results:

Real-time PCR detected fungal nucleic acid in 51% of samples, compared to 39% by culture.
Identified 91% as T. rubrum and 8% as T. interdigitale among PCR-positive samples.
The PCR method demonstrated higher sensitivity and was 2-4 weeks faster than culture.

Conclusions:

The developed real-time PCR is a sensitive and rapid diagnostic tool for dermatophytosis.
This method allows for faster identification of key dermatophyte species.
Offers a significant improvement over conventional culture methods for clinical diagnosis.