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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 14, 2026

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Published on: August 26, 2021

Measuring apoptosis by microscopy and flow cytometry.

Conor M Henry¹, Emilie Hollville, Seamus J Martin

¹Molecular Cell Biology Laboratory, Department of Genetics, The Smurfit Institute, Trinity College, Dublin 2, Ireland.

Methods (San Diego, Calif.)

|February 14, 2013

Summary

This summary is machine-generated.

Apoptosis is programmed cell death with specific cellular changes, unlike necrosis which causes inflammation. This study reviews assays to distinguish these cell death types.

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Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

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Published on: August 26, 2021

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Published on: November 17, 2011

Area of Science:

Cell Biology
Biochemistry
Immunology

Background:

Apoptosis is programmed cell death involving cellular shrinkage, blebbing, and DNA fragmentation.
Necrosis is uncontrolled cell death characterized by swelling and membrane rupture, often triggering inflammation.
Distinguishing apoptosis from necrosis is crucial due to their differing physiological and pathological implications.

Purpose of the Study:

To differentiate apoptosis from necrosis.
To highlight assays for identifying cell death modes.

Main Methods:

Microscopy techniques.
Flow cytometry analysis.

Main Results:

Apoptotic cells exhibit distinct morphological and biochemical markers.
Necrotic cells release danger signals, inducing inflammation.
Assays can reliably distinguish between apoptotic and necrotic cell death.

Conclusions:

Accurate identification of cell death pathways is essential.
Microscopy and flow cytometry are valuable tools for assessing apoptosis versus necrosis.