Inhibitory effects of technical grade methoxychlor on development of neonatal male mouse reproductive organs

Effects of technical grade methoxychlor (MX), an estrogenic insecticide, and 17 beta-estradiol (E2) were examined on serum testosterone (T) concentrations and growth and histology of neonatal male mouse reproductive organs. Male NIH/Swiss mice received i.p. injections daily from birth to Day 9 with one of the following: 10 micrograms E2 or 0.1 or 1.0 mg MX or sesame oil vehicle. The mice were killed on Day 10. MX or did not affect body weights or mortality. Serum T concentrations in control mice were 0.29 +/- 0.03 ng/ml; T concentrations in males treated with MX or E2 were reduced. The 1.0-mg dose of both MX and E2 significantly decreased DNA contents of the seminal vesicles (SV), bulbourethral glands (BUG), and ventral prostate (VP) compared to controls. In the same animals, DNA contents of testes, epididymides, and efferent ductules were not decreased. The lower dose of MX (0.1 mg) decreased DNA content of only BUG and SV. DNA content of the ductus deferens was not affected by any treatment. E2 and both doses of MX also decreased epithelial morphogenesis in the SV and BUG, and inhibited the onset of mucin production in BUG epithelium and smooth muscle differentiation in the ductus deferens. In summary, our results indicate that technical grade MX, at doses as low as 0.1 mg/day, and E2 inhibit neonatal male reproductive tract development and decrease serum T concentrations.