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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 14, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
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Published on: August 2, 2018

Technologies and challenges in large-scale phosphoproteomics.

Kasper Engholm-Keller1, Martin R Larsen

  • 1Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.

Proteomics
|February 14, 2013
PubMed
Summary
This summary is machine-generated.

Phosphorylation regulates key cellular processes and is vital in disease. This review covers mass spectrometry-based phosphoproteomics methods, challenges, and limitations for analyzing protein phosphorylation.

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Last Updated: May 14, 2026

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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

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Area of Science:

  • Biochemistry and Molecular Biology
  • Proteomics
  • Cellular Signaling

Background:

  • Phosphorylation, a reversible protein modification, is crucial for regulating protein activity, stability, and interactions.
  • This post-translational modification (PTM) underpins essential cellular functions including signaling, synthesis, degradation, and apoptosis.
  • Dysregulation of phosphorylation is implicated in numerous diseases, highlighting the importance of its study.

Purpose of the Study:

  • To review state-of-the-art mass spectrometry (MS)-based methods for analyzing protein phosphorylation.
  • To discuss strategies for large-scale phosphoproteomic experiments.
  • To identify current challenges and limitations in the field of phosphoproteomics.

Main Methods:

  • Mass spectrometry (MS)-based phosphoproteomics for comprehensive identification of phosphorylation sites.
  • Techniques for analyzing protein phosphorylation in various biological contexts (cells, tissues, organisms).
  • Strategies for non-hypothesis-driven discovery and characterization of phosphoproteins.

Main Results:

  • Detailed overview of current MS-based analytical methods for protein phosphorylation.
  • Discussion of experimental strategies for large-scale phosphoproteomic studies.
  • Identification of key challenges and limitations in current phosphoproteomics research.

Conclusions:

  • MS-based phosphoproteomics is the primary technique for discovering and characterizing phosphoproteins.
  • Understanding phosphorylation dynamics is critical for deciphering cellular processes and disease mechanisms.
  • Addressing current challenges is essential for advancing the field of phosphoproteomics.