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Related Experiment Video

Updated: May 14, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Micro-/nanofluidics based cell electroporation.

Shengnian Wang¹, L James Lee

¹Institute for Micromanufacturing, Louisiana Tech University, Ruston, Louisiana 71272, USA and Chemical Engineering Program, Louisiana Tech University, Ruston, Louisiana 71272, USA.

Biomicrofluidics

|February 14, 2013

Summary

This summary is machine-generated.

Micro-/nanofluidic electroporation offers a powerful, cell-friendly alternative to viral gene delivery. These advanced systems enhance transfection efficiency and control for diverse biological and medical applications.

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Last Updated: May 14, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

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Published on: January 7, 2022

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

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Published on: August 7, 2014

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

Area of Science:

Biotechnology
Bioengineering
Molecular Biology

Background:

Non-viral gene delivery is crucial for replacing viral systems.
Electroporation is a versatile non-viral method with no cell or probe restrictions.
Micro-/nanofluidics have emerged as advanced platforms for cell electroporation.

Purpose of the Study:

To summarize advances in micro-/nanofluidics-based cell electroporation.
To highlight advantages over conventional bulk electroporation systems.
To discuss future research directions for broader applications.

Main Methods:

Review of micro-/nanofluidic technologies for cell electroporation.
Analysis of gene delivery performance for various molecules (DNA, RNA, nanoparticles).
Examination of challenges and solutions in microfluidic electroporation design.

Main Results:

Micro-/nanofluidic electroporation offers high pulse strength at low voltage.
Enables precise control over cell/probe concentration and positioning.
Facilitates real-time, single-cell monitoring of intracellular trafficking.
Demonstrates flexibility for transfecting single cells to large populations.

Conclusions:

Micro-/nanofluidic electroporation significantly improves upon bulk systems.
Addresses key challenges like electrochemical reactions and cell viability.
Holds great potential for advancing life sciences and medical applications.