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Related Experiment Video

Updated: May 14, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

[Cigarette smoking affects sperm plasma membrane integrity].

Wei-Wei Li¹, Na Li, Qiu-Yue Wu

¹Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, China.

Zhonghua Nan Ke Xue = National Journal of Andrology

|February 15, 2013

Summary

This summary is machine-generated.

Cigarette smoking significantly impairs sperm plasma membrane integrity (PMI) and motility in infertile men. SYBR-14/PI staining and flow cytometry accurately detect these changes, highlighting smoking as a key factor in male infertility.

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Last Updated: May 14, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes

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Published on: November 28, 2018

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

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Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

Published on: February 21, 2011

Area of Science:

Reproductive biology and toxicology
Spermatozoa function assessment
Flow cytometry applications in andrology

Context:

Male infertility is a significant concern, with lifestyle factors like smoking playing a crucial role.
Assessing sperm plasma membrane integrity (PMI) is vital for evaluating male fertility.
Current methods for PMI assessment can be time-consuming or lack precision.

Purpose:

To evaluate sperm plasma membrane integrity (PMI) in infertile men who smoke using SYBR-14/PI fluorescent staining and flow cytometry.
To investigate the clinical significance of these findings in relation to cigarette consumption levels.
To establish a rapid and accurate method for detecting sperm damage.

Summary:

Semen samples from 132 infertile male smokers (heavy and light) and 70 fertile controls were analyzed.
Sperm PMI was determined via flow cytometry using SYBR-14/PI staining, differentiating live, dead, and moribund sperm.
Both smoker groups exhibited significantly altered PMI compared to controls, with heavy smokers showing greater impairment.

Impact:

SYBR-14/PI staining coupled with flow cytometry provides a quick and precise method for detecting sperm PMI.
Cigarette smoking demonstrably reduces sperm PMI and motility, contributing significantly to male infertility.
This technique can aid in diagnosing and managing male infertility linked to smoking.