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Analyzing arthropods for the presence of bacteria
1Department of Entomology, University of Kentucky, Lexington, Kentucky, USA.
Current Protocols in Microbiology
|February 15, 2013
Abstract:
Bacteria within arthropods can be identified using culture-independent methods. This unit describes protocols for surface sterilization of arthropods, DNA extraction of whole bodies and tissues, touchdown PCR amplification using 16S rDNA general bacteria primers, and profiling the bacterial community using denaturing gradient gel electrophoresis.

