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Related Experiment Video

Updated: May 14, 2026

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
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Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

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Human immunodeficiency viruses: propagation, quantification, and storage.

Paul J Peters1, Kathryn Richards, Paul Clapham

  • 1Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.

Current Protocols in Microbiology
|February 15, 2013
PubMed
Summary
This summary is machine-generated.

This unit details essential protocols for human immunodeficiency virus (HIV) research, covering propagation, quantification, and storage methods. It also provides background, advantages, disadvantages, and troubleshooting for these key HIV research techniques.

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Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Human Immunodeficiency Virus (HIV) research requires standardized and reliable laboratory protocols.
  • Understanding the advantages and disadvantages of different methods is crucial for efficient research.
  • Troubleshooting common issues ensures the integrity of experimental results.

Purpose of the Study:

  • To provide fundamental protocols for human immunodeficiency virus (HIV) research.
  • To offer methods for propagating and quantifying HIV.
  • To give recommendations for the long-term storage of HIV samples and reagents.

Main Methods:

  • Detailed protocols for HIV propagation.
  • Methods for accurate HIV quantification.
  • Guidelines for optimal long-term HIV sample storage.

Main Results:

  • Established procedures for culturing and amplifying HIV.
  • Validated techniques for measuring HIV levels.
  • Best practices for preserving HIV integrity during storage.

Conclusions:

  • Standardized protocols are vital for reproducible HIV research.
  • Effective propagation, quantification, and storage methods enhance research efficiency.
  • This unit serves as a practical guide for researchers working with HIV.